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The development of naive T cells into peripheral dendritic cells (DC) has been demonstrated in several organs such as the thymus, skin and mucosa. However, there is not much known about the ontogeny of peripheral DCs and their regulation. Furthermore, how T cells initiate the migratory process that leads to the generation of peripheral DCs is still unclear. To investigate this, we analyzed in vivo the development of DCs under different conditions of T cell activation and the role of the L-selectin (CD62L)-mediated cell adhesion. Treatment of mice with antibodies to CD62L (RP105-1) interfered with the generation of DCs in the mucosa-associated lymphoid tissue (MALT) and intestinal Peyer’s patches (PP). In contrast, transfer of CD4(+)CD62L(-) T cells resulted in a significant accumulation of DCs in the gut-associated lymphoid tissue (GALT). In situ hybridization of in vitro developing cell lines demonstrated that CD62L-mediated T cell-DC migratory signals act in the first step of DC generation, whereas the second step is controlled by the homeostatic chemokine receptor, CCR7.Q:
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